Urinary excretion of biopyrrin in unconjugated hyperbilirubinemia
نویسندگان
چکیده
Biopyrrin is an oxidative metabolite of bilirubin. Biopyrrin is a tripyrrol moiety of bilirubin, including two regioisomers (biotripyrrin-a, 1,14,15,17tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16Htripyrrin-8,12-dipropionic acid and biotripyrrin-b, 1,14,15,17-tetrahydro-3,7,13-trimethyl-1,14-deoxy2-vinyl-16H-tripyrrin-8,12-dipropionic acid: Fig. 1). For many years, bilirubin was regarded as a potentially toxic waste product formed during heme catabolism. However, recent evidence suggests that bilirubin is a physiological antioxidant that may scavenge oxygen and peroxyl radicals as efficiently asα-tocopherol. Circulating bilirubin functions as a suicide antioxidant; it is catabolized to biopyrrin and is excreted in urine. We now have an assay method for biopyrrin in urine that uses an enzyme-linked immunosorbent assay (ELISA) and an anti-bilirubin monoclonal antibody, 24G7. The antibody has an epitope in the dipyrrole Journal of Analytical Bio-Science Vol. 30, No 4 (2007)
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